Seralini and Science: an Open Letter
Independent Science News, October 2, 2012
http://independentsciencenews.org/health/seralini-and-science-nk603-rat-study-roundup/#comment-4292
(For a full list of all the signatories, and hyperlinkis to many of the cited references, please go to the URL above)
A new paper by the French group of Gilles-Eric Seralini describes harmful effects on rats fed diets containing genetically modified maize (variety NK603), with and without the herbicide Roundup, as well as Roundup alone. This peer-reviewed study (Seralini et al., 2012), has been criticized by some scientists whose views have been widely reported in the popular press (Carmen, 2012; Mestel, 2012; Revkin, 2012; Worstall, 2012). Seralini et al. (2012) extends the work of other studies demonstrating toxicity and/or endocrine-based impacts of Roundup (Gaivão et al., 2012; Kelly et al., 2010; Paganelli et al., 2010; Romano et al., 2012), as reviewed by Antoniou et al. (2010).
The Seralini publication, and resultant media attention, raise the profile of fundamental challenges faced by science in a world increasingly dominated by corporate influence. These challenges are important for all of science but are rarely discussed in scientific venues.
1) History of Attacks on Risk-finding Studies. Seralini and colleagues are just the latest in a series of researchers whose findings have triggered orchestrated campaigns of harassment. Examples from just the last few years include Ignacio Chapela, a then untenured Assistant Professor at Berkeley, whose paper on GM contamination of maize in Mexico (Quist and Chapela, 2001) sparked an intensive internet-based campaign to discredit him. This campaign was reportedly masterminded by the Bivings Group, a public relations firm specializing in viral marketing – and frequently hired by Monsanto (Delborne, 2008).
The distinguished career of biochemist Arpad Pusztai, came to an effective end when he attempted to report his contradictory findings on GM potatoes (Ewen and Pusztai, 1999a). Everything from a gag order, forced retirement, seizure of data, and harassment by the British Royal Society were used to forestall his continued research (Ewen and Pusztai, 1999b; Laidlaw, 2003). Even threats of physical violence have been used, most recently against Andres Carrasco, Professor of Molecular Embryology at the University of Buenos Aires, whose research (Paganelli et al. 2010) identified health risks from glyphosate, the active ingredient in Roundup (Amnesty International, 2010).
It was no surprise therefore, that when in 2009, 26 corn entomologists took the unprecedented step of writing directly to the US EPA to complain about industry control of access to GM crops for research, the letter was sent anonymously (Pollack, 2009).
2) The Role of the Science Media. An important but often unnoticed aspect of this intimidation is that it frequently occurs in concert with the science media (Ermakova, 2007; Heinemann and Traavik, 2007; Latham and Wilson, 2007). Reporting of the Seralini paper in arguably the most prestigious segments of the science media: Science, the New York Times, New Scientist, and the Washington Post uniformly failed to "balance" criticism of the research, with even minimal coverage of support for the Seralini paper (Carmen, 2012; Enserink, 2012; MacKenzie, 2012; Pollack, 2012). Nevertheless, less well-resourced media outlets, such as the UK Daily Mail appeared to have no trouble finding a positive scientific opinion on the same study (Poulter, 2012).
3) Misleading Media Reporting. A key pattern with risk-finding studies is that the criticisms voiced in the media are often red herrings, misleading, or untruthful. Thus, the use of common methodologies was portrayed as indicative of shoddy science when used by Seralini et al. (2012) but not when used by industry (see refs above and Science Media Centre, 2012). The use of red herring arguments appears intended to sow doubt and confusion among non-experts. For example, Tom Sanders of Kings College, London was quoted as saying: "This strain of rat is very prone to mammary tumors particularly when food intake is not restricted" (Hirschler and Kelland, 2012 ). He failed to point out, or was unaware, that most industry feeding studies have used Sprague-Dawley rats (e.g. Hammond et al., 1996, 2004, 2006; MacKenzie et al., 2007). In these and other industry studies (e.g. Malley et al. 2007), feed intake was unrestricted. Sanders' comments are important because they were widely quoted and because they were part of an orchestrated response to the Seralini study by the Science Media Centre of the British Royal Institution. The Science Media Centre has a long history of quelling GMO controversies and its funders include numerous companies that produce GMOs and pesticides.
4) Regulator Culpability. In our view a large part of the ultimate fault for this controversy lies with regulators. Regulators, such as EFSA (the European Food Safety Authority) in Europe and the EPA (Environmental Protection Agency) and FDA (Food and Drug Administration) in the US, have enshrined protocols with little or no potential to detect adverse consequences of GMOs (Schubert, 2002; Freese and Schubert, 2004; Pelletier, 2005).
GMOs are required to undergo few experiments, few endpoints are examined, and tests are solely conducted by the applicant or their agents. Moreover, current regulatory protocols are simplistic and assumptions-based (RSC, 2001), which by design, will miss most gene expression changes – apart from the target trait - induced by the process of transgene insertion (Heinemann et al., 2011; Schubert, 2002).
Puzstai (2001) and others have consequently argued that well-conducted feeding trials are one of the best ways of detecting such unpredictable changes. Yet feeding trials are not mandatory for regulatory approval, and the scientific credibility of those which have been published to date has been challenged (Domingo, 2007; Pusztai et al., 2003; Spiroux de Vendômois et al., 2009). For example, Snell et al. (2012), who assessed the quality of 12 long term (>96 days) and 12 multigenerational studies, concluded: "The studies reviewed here are often linked to an inadequate experimental design that has detrimental effects on statistical analysis…the major insufficiencies not only include lack of use of near isogenic lines but also statistical power underestimation [and], absence of repetitions…".
Apparently, the same issues of experimental design and analysis raised about this (Seralini) risk-finding study were not of concern to critics when the studies did not identify risk, resulting in ill-informed decision-makers. In the end, it is a major problem for science and society when current regulatory protocols approve GMO crops based on little to no useful data upon which to assess safety.
5) Science and Politics. Governments have become habituated to using science as a political football. For example, in a study conducted by the Royal Society of Canada at the request of the Canadian government, numerous weaknesses of GM regulation in Canada were identified (RSC, 2001). The failure of the Canadian government to meaningfully respond to the many recommended changes was detailed by Andree (2006). Similarly, the expert recommendations of the international IAASTD report, produced by 400 researchers over 6 years, that GMOs are unsuited to the task of advancing global agriculture have been resolutely ignored by policymakers. Thus, while proclaiming evidence-based decision-making, governments frequently use science solely when it suits them.
6) Conclusion: When those with a vested interest attempt to sow unreasonable doubt around inconvenient results, or when governments exploit political opportunities by picking and choosing from scientific evidence, they jeopardize public confidence in scientific methods and institutions, and also put their own citizenry at risk. Safety testing, science-based regulation, and the scientific process itself, depend crucially on widespread trust in a body of scientists devoted to the public interest and professional integrity. If instead, the starting point of a scientific product assessment is an approval process rigged in favour of the applicant, backed up by systematic suppression of independent scientists working in the public interest, then there can never be an honest, rational or scientific debate.
The Authors: Susan Bardocz (4, Arato Street, Budapest, 1121 Hungary); Ann Clark (University of Guelph, ret.); Stanley Ewen (Consultant Histopathologist, Grampian University Hospital); Michael Hansen (Consumers Union); Jack Heinemann (University of Canterbury); Jonathan Latham (The Bioscience Resource Project); Arpad Pusztai (4, Arato Street, Budapest, 1121 Hungary); David Schubert (The Salk Institute); Allison Wilson (The Bioscience Resource Project)
Footnotes
(1) In addition, US scientists who publish studies finding adverse environmental effects are frequently vehemently attacked by other pro-GM scientists. As a report in Nature, which discusses numerous examples, points out, "Papers suggesting that biotech crops might harm the environment attract a hail of abuse from other scientists. Behind the attacks are scientists who are determined to prevent papers they deem to have scientific flaws from influencing policy-makers. When a paper comes out in which they see problems, they react quickly, criticize the work in public forums, write rebuttal letters, and send them to policy-makers, funding agencies and journal editors" (pg. 27 in Waltz. 2009a. Indeed, when one of us wrote a Commentary in Nature Biotechnology ten years ago suggesting that more attention needs to be paid to the potential unintended effects associated with insertional mutagenesis, we received a flood of responses, and an administrator at the Salk Institute even said that the publication "was jeopardizing funding for his institution" (see Waltz, 2009a). Similar attacks have greeted studies on adverse effects of Bt toxins on ladybird beetles and green lacewing larvae, which were used by German authorities to ban cultivation of Mon810, a Bt corn variety (see: Hilbeck et al. 2012a,b , respectively). In 2009, a group of 26 public sector corn entomologists sent a letter to the US Environmental Protection Agency which stated "No truly independent research can be legally conducted on many critical questions involving these crops [because of company-imposed restrictions]" (pg. 880 in Waltz, 2009b; it was no surprise that the letter was sent anonymously as the scientists feared retribution from the companies that funded their work (Pollack, 2009). Furthermore, industry control over what research can be conducted in the US means that adverse findings can effectively be suppressed. In one example cited in the article, Pioneer was developing a binary Bt toxin, Cry34Ab1/Cry35Ab1, against the corn rootworm. In 2001, Pioneer contracted with some university laboratories to test for unintended effects on a lady beetle. The laboratories found that 100% of the lady beetles died after eight days of feeding. Pioneer forbade the researchers from publicizing the data. Two years later Pioneer received approval for a Bt corn variety with Cry34Ab1/Cry35Ab1 and submitted studies showing that lady beetles fed the toxin for only 7 days were not harmed. The scientists were not allowed to redo the study after the crop was commercialized (Waltz, 2009b). In another example, Dow AgroSciences threatened a researcher with legal action if he published information he had received from US EPA. As the article notes, "The information concerned an insect-resistant variety of maize known as TC1507, made by Dow and Pioneer. The companies suspended sales of TC1507 in Puerto Rico after discovering in 2006 that an armyworm had developed resistance to it. Tabashnik was able to review the report the companies filed with the EPA by submitting a Freedom of Information Act request. "I encouraged an employee of the company [Dow] to publish the data and mentioned that, alternatively, I could cite the data," says Tabashnik. "He told me that if I cited the information…I would be subject to legal action by the company," he says. "These kinds of statements are chilling" (pg. 882 in Waltz, 2009b).
References
Amnesty International. 2010. Argentina: Threats deny community access to research UA: 173/10 Index: AMR 13/005/2010 Argentina Date: 12 August 2010
Andree, Peter. 2006. An analysis of efforts to improve genetically modified food regulation in Canada. Science and Public Policy 33(5):399-389.
Antoniou, Michael., Paolo Brack, Andres Carrasco, John Fagan, Mohamed Habib, Paolo Kageyama, Carlo Leifert, Rubens Nodari, Walter Pengue. 2010. GM Soy: Sustainable? Responsible? GLS Gemeinschaftsbank and ARGE Gentechnik-frei.
Carmen, Tim. 2012. French scientists question safety of GM corn. Washington Post 19 Sept 2012.
Delborne, Jason. 2008. Transgenes and transgressions: scientific dissent as heterogeneous practice. Social Studies of Science 38(4):509-541
Domingo, Jose L. 2007. Toxicity studies of genetically modified plants: a review of the published literature. Crit. Rev. Food Sci. Nutr. 47:721–733
Enserink, Martin. 2012. France and European Commission order review of controversial GM study in rats. ScienceInsider 21 Sept 2012
Ermakova, Irina. 2007. GM soybeans: revisiting a controversial format. Nature Biotech 25:1351-1354
Ewen, Stanley W.B. and Arpad Pusztai. 1999a Effect of diets containing genetically modified potatoes expressing Galanthus nivalis lectin on rat small intestine. The Lancet 354 (9187):1353-1354
Ewen, Stanley W.B. and Arpad Pusztai. 1999b. Health risks of genetically modified foods. The Lancet 354(Issue 9179):684. HYPERLINK "
Freese, W. and D. Schubert. 2004. Safety Testing and Regulation of Genetically Engineered Foods. Biotechnol Genet Eng Rev 21:299-324
Gaivão I, Guilherme S, M.A. Santos MA, M. Pacheco. 2012. DNA damage in fish (Anguilla anguilla) exposed to a glyphosate-based herbicide — elucidation of organ-specificity and the role of oxidative stress. Mutat Res 18;743(1-2):1-9.
Hammond, Bruce, John L Vicini, Cary F. Hartnell, Mark W. Naylor, Christopher D. Knight, Edwin H. Robinson, Roy L. Fuchs and Stephen R. Padgette. 1996. The feeding value of soybeans fed to rats, chickens, catfish and dairy cattle is not altered by genetic incorporation of glyphosate tolerance. J. Nutr. 126:717-272
Hammond, B., R. Dudek, J. Lemen, M. Nemeth. 2004. Results of a 13 week safety assurance study with rats fed grain from glyphosate tolerant corn. Food Chem Toxicol 42:1003–1014
Hammond, B., R. Dudek, J. Lemen, M. Nemeth. 2006. Results of a 90-day safety assurance study with rats fed grain from corn borer-protected corn. Food Chem Toxicol 44:1092–1099
Heinemann, J.A. and Traavik, T. 2007. GM soybeans—revisiting a controversial format. Nature Biotech 25: 1355-1356
Heinemann, J. A., B. Kurenbach, B. and D. Quist. 2011. Molecular profiling — a tool for addressing emerging gaps in the comparative risk assessment of GMOs. Env. Int. 37: 1285-1293.
Hilbeck, A., J.M. McMillan, M. Meier, A. Humbel, J. Schläpfer-Miller and M. Trtikova. 2012. A controversy revisited: Is the coccinellid Adalia bipunctata adversely affected by Bt toxins? Environmental Sciences Europe, 24:10
Hilbeck, A., M. Meier and M. Trtikova. 2012. Underlying reasons of the controversy over adverse effects of Bt toxins on lady beetle and lacewing larvae. Environmental Sciences Europe, 24:9.
Hirschler, Ben and Kate Kelland. 2012. Study on Monsanto GM corn concerns draws skepticism. Reuters: Ed UK 20 Sept 2012
Kelly, David, Robert Poulin, Daniel M. Tompkins and Colin R. Townsend. 2010. Synergistic effects of glyphosate formulation and parasite infection on fish malformations and survival. J. Appl. Ecol. 47(2): 498-504
Laidlaw, Stuart. 2003. Ch. 4 What's Good for GM. In: Secret Ingredients. McClelland and Stewart Ltd., Toronto.
Latham, Jonathan and Allison Wilson. 2007. What is Nature Biotechnology good for? Independent Science News 4 Dec 2007.
MacKenzie, Debora. 2012. Study linking GM crops and cancer questioned. New Scientist 19 Sept 2012.
MacKenzie and 12 others. 2007. Thirteen week feeding study with transgenic maize grain containing event DAS-Ø15Ø7-1 in Sprague–Dawley rats. Food Chem. Toxicol. 45:551–562
Malley and 14 others. 2007. Subchronic feeding study of DAS-59122-7 maize grain in Sprague-Dawley rats. Food Chem. Toxicol. 45:1277–1292
Mestel, Rosie. 2012. Study points to health problems with genetically modified foods. LA Times 20 Sept 2012
Paganelli, Alejandra, Victoria Gnazzo, Helena Acosta, Silvia L. Lopez, and Andres E. Carrasco. 2010. Glyphosate-based herbicides produce teratogenic effects on vertebrates by impairing retinoic acid signaling. Chem. Res. Toxicol. 23(10):1586–1595
Pelletier, D. 2005. Science, Law, and Politics in the Food and Drug Administration's Genetically Engineered Foods Policy: FDA's 1992 Policy Statement. Nutr. Rev. 63:171-181
Pollack, Andrew. 2009. Crop scientists say biotechnology seed companies are thwarting research. New York Times 19 Feb 2009.
Pollack, Andrew. 2012. Foes of modified corn find support in a study. New York Times 19 Sept 2012.
Poulter, Sean. 2012. Cancer row over GM foods as study says it did THIS to rats… and can cause organ damage and early death in humans. Mail OnLine 19 Sept 2012.
Pusztai, Arpad. 2001. Genetically Modified Foods: Are They a Risk to Human/Animal Health? American Institute of Biological Sciences
Pusztai, A,, S. Bardocz, and S.W.B. Ewen. 2003. Ch. 16. Genetically Modified Foods: Potential Human Health Effects. pp. 347-372. In: J.P.F. D'Mello (ed) Food Safety: Contaminants and Toxins. CAB International 472 pp.
Quist, David and Ignacio Chapela. 2001. Transgenic DNA introgressed into traditional maize landraces in Oaxaca, Mexico. Nature 414 (6863): 541–543
Revkin, Andrew. 2012. Single-Study Syndrome and the G.M.O. Fight. New York Times 20 Sept 2012 HYPERLINK "" http://dotearth.blogs.nytimes.com/2012/09/20/the-gmo-food-fight-rats-cancer-and-single-study-syndrome/
Romano M.A., R.M. Romano, L.D. Santos, P. Wisniewski, D.A. Campos, P.B. de Souza, P. Viau, M.M. Bernardi, M.T. Nunes, C.A. de Oliveira. 2012. Glyphosate impairs male offspring reproductive development by disrupting gonadotropin expression. Arch. Toxicol. 86(4):663-73.
RSC (Royal Society of Canada). 2001. Elements of Precaution: Recommendations for the Regulation of Food Biotechnology In Canada
Science Media Centre. 2012. Study on cancer and GM maize – experts respond. Posted 20 Sept 2012.
Schubert, David. 2002. A different perspective on GM food. Nature Biotech. 20: 969
Séralini, G-E., E. Clair, R. Mesnage, S. Gress, N. Defarge, M. Malatesta, D. Hennequin, J. Spiroux de Vendômois. 2012. Long term toxicity of a Roundup herbicide and a Roundup-tolerant genetically modified maize. Food Chem. Toxicol.
Snell, C., A. Bernheim, J-B. Berge, M. Kuntz, G. Pascal, A. Paris, and A.E. Ricroch. 2012. Assessment of the health impact of GM plant diets in long-term and multigenerational animal feeding trials: A literature review. Food Chem. Toxicol. 50:1134-1148
Spiroux de Vendômois, J., F. Roullier, D. Cellier, and G.-E. Séralini. 2009. A Comparison of the Effects of Three GM Corn Varieties on Mammalian Health. Int. J. Biol. Sci. 5(7):706–726
Waltz, E. 2009a. Battlefield. Nature 461: 27-32.
Waltz, E. 2009b. Under Wraps. Nature Biotechnology 27(10): 880-882.
Worstall, Tim. 2012. Proof Perfect That The Seralini Paper On GM Corn And Cancer In Rats Is Rubbish. Forbes 21 Sept 2012.