GM Free Cymru

Crucial paper 60: MON810 effects on Daphnia magna show it is not substantially equivalent

Date Added to website 18th August 2015

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Crucial paper 60: MON810 effects on Daphnia magna show it is not substantially equivalent
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Chronic Responses of Daphnia magna Under Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt-Maize Hybrid and its Conventional Near-Isoline.
Ferreira Holderbaum, Cuhra M, Wickson, Orth A, Nodari RO, Bøhn T.
J Toxicol Environ Health A. 2015;78(15):993-1007.
doi: 10.1080/15287394.2015.1037877.

Abstract
Insect resistance is the second most common trait globally in cultivated genetically modified (GM) plants. Resistance is usually obtained by introducing into the plant's genome genes from the bacterium Bacillus thuringiensis (Bt) coding for insecticidal proteins (Cry proteins or toxins) that target insect pests. The aim of this study was to examine the hypothesis that a chronic, high-dose dietary exposure to leaves of a Bt-maize hybrid(GM event MON810, expressing a transgenic or recombinant Cry1Ab toxin), exerted no adverse effects on fitness parameters of the aquatic nontarget organism Daphnia magna (water flea) when compared to an identical control diet based on leaves of the non-GM near-isoline. Cry1Ab was immunologically detected and quantified in GM maize leaf material used for Daphnia feed. A 69-kD protein near Bt's active core-toxin size and a 34-kD protein were identified. The D. magna bioassay showed a resource allocation to production of resting eggs and early fecundity in D. magna fed GM maize, with adverse effects for body size and fecundity later in life. This is the first study to examine GM-plant leaf material in the D. magnamodel, and provides of negative fitness effects of a MON810 maize hybrid in a nontarget model organism under chronic, high dietary exposure. Based upon these results, it is postulated that the observed transgenic proteins exert a nontarget effect in D. magna and/or unintended changes were produced in the maize genome/metabolome by the transformation process, producing a nutritional difference between GM-maize and non-GMnear-isoline.
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Long-term exposure to GM maize leaves harms water fleas
http://us6.campaign-archive2.com/?u=29cbc7e6c21e0a8fd2a82aeb8&id=3753f622cc&e=2350a21e83

New study confirms GM maize MON810 is not substantially equivalent to non-GM maize

Long-term exposure to leaves of MON810 GM Bt maize causes reduced growth and fertility in Daphnia magna (water flea), compared with the level of the non-GM parent variety (isoline), a new study has found. The authors suggest that the transgenic protein and/or unintended changes in the maize due to the GM process were the causes of the observed effects.

Daphnia magna is an important indicator species for environmental toxicity.

The study shows that this GM maize is not substantially or biologically equivalent to the non-GM parent variety (yet it was approved by regulators worldwide on the basis that it is) and is more toxic.

In addition, unique characteristics were found in the GM Cry1Ab insecticidal toxin of MON810 Bt maize, as opposed to bacterially produced Cry1Ab. Yet bacterially produced Bt toxins are routinely used in many regulatory tests to assess the safety of GM Bt crops. The authors call for the use of Bt toxins as actually produced in the plant in regulatory tests.